Preparation and qualification of internal rabies reference standards for use in the rabies rapid fluorescent focus inhibition test
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چکیده
منابع مشابه
Comparison of a Micro-Neutralization Test with the Rapid Fluorescent Focus Inhibition Test for Measuring Rabies Virus Neutralizing Antibodies.
The rapid fluorescent focus inhibition test (RFFIT) is routinely used in the United States to measure rabies virus neutralizing antibodies (rVNA). RFFIT has a long history of reproducible and reliable results. The test has been modified over the years to use smaller volumes of reagents and samples, but requires a 50 μL minimum volume of test serum. To conduct pathogenesis studies, small laborat...
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Inactivation of rabies virus in reagents used for the fluorescent rabies antibody test.
Procedures for inactivating rabies virus in reagents used for the fluorescent rabies antibody test are described. Mouse brain adsorbing suspensions containing greater than or equal to 10(9) 50% lethal doses of virus per ml were rendered noninfectious by treatment with 0.1% beta-propiolactone or by heating at 56 degrees for greater than or equal to 30 min. Viable virus in tissue impression smear...
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A prospective study of 8,987 canine, feline, human, and other mammalian brains (and one avian brain) was undertaken. The brains were analyzed for rabies antigens; two samples were obtained from each brainstem, and one sample was obtained from each hippocampus. The samples were stained with rabies conjugate and examined by use of fluorescence microscopy. There were no false-negative results. We ...
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19 The antemortem diagnosis of rabies in humans employs techniques that require 20 accuracy, speed and sensitivity. A combination of histochemical analysis, in vitro virus isolation, 21 immunologic methods and molecular amplification procedures are utilized in an effort to 22 diagnose the disease. Modern medicine now offers potentially life-saving treatment for a disease 23 that was considered ...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-66754-8